High-throughput method to analyze the cytotoxicity of CAR T Cells in a 3D tumor spheroid model using image cytometry

Abstract Chimeric antigen receptor (CAR)-T cell therapy is an antigen-dependent cellular that has gained considerable traction in the field of cancer immunotherapy. Currently, there are six FDA-approved CAR-T therapies, which all target CD19 or BCMA antigens for hematologic B malignancies. In recent years, a strong focus been placed on CAR T discovery solid tumors, may better recapitulate physiological conditions, thereby potentially improving selection construct candidates. Therefore, it critical to develop novel methodologies high-throughput phenotypic and functional assays using 3D tumor spheroid models assess therapies against tumors. Recently, plate-based image cytometry emerged as method investigate characterize functions manner. Image demonstrated capabilities analyzing transduction efficiency, proliferation, cytotoxicity therapy. With development models, provide necessary tools applications geared towards this work, we discuss use cells targeted PSMA, found prostate cells, second most common cause deaths among men worldwide. Herein, demonstrate PSMA cell-mediated cytotoxic potency spheroids simultaneously monitor location vitro. The proposed can effectively generate time- dose-dependent results.